Arginase I activity remained unchanged during aging in HC. RBC-NOS Serine 1177 phosphorylation, indicating enzyme activation, increased during aging in both HC and DM. RBC deformability was lower in DM and significantly decreased in old compared to young RBC in both HC and DM. RBC nitrite, as marker for NO, was higher in DM and increased in both HC and DM during aging. In both groups, in vivo aging was marked by changes in RBC shape and decreased cell volume. The proportion of old RBC was significantly higher in DM compared to HC.
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